Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Gonad
Cell type
SCCOHT-1
NA
NA

Attributes by original data submitter

Sample

source_name
SCCOHT-1 Ovarian Cancer Cell Line
cell line
SCCOHT-1
transfected vector
Control
antibody
Brg1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Samples were processed for CUT&RUN following protocol from https://dx.doi.org/10.17504/protocols.io.zcpf2vn . Antibodies were used at 1:25 dilution . Libraries were construct using Kapa Hyperprep DNA kit and Kapa dual index adapters per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
25111349
Reads aligned (%)
72.0
Duplicates removed (%)
61.4
Number of peaks
249 (qval < 1E-05)

hg19

Number of total reads
25111349
Reads aligned (%)
71.1
Duplicates removed (%)
62.6
Number of peaks
219 (qval < 1E-05)

Base call quality data from DBCLS SRA